Interleukin-15-induced CD56(+) myeloid dendritic cells combine potent tumor antigen presentation with direct tumoricidal potential

摘要: Dendritic cells (DCs) are the quintessential antigen-presenting of human immune system and play a prime role in coordinating innate adaptive responses, explaining strong still growing interest their application for cancer immunotherapy. Much current research field DC-based immunotherapy focuses on optimizing culture conditions vitro DC generation order to assure that DCs with best possible immunogenic qualities being used In this context, monocyte-derived alternatively induced by interleukin-15 (IL-15 DCs) have attracted recent attention due superior immunostimulatory characteristics. study, we show IL-15 DCs, addition potent tumor function, possess tumoricidal potential thus qualify designation killer DCs. Notwithstanding marked expression natural (NK) cell marker CD56 subset found no evidence further phenotypic overlap between NK cells. Allostimulation antigen presentation assays confirmed should be regarded as bona fide myeloid not only from but also functional point view. Concerning cytotoxic activity, demonstrate able induce apoptotic death K562 line, while sparing antigen-specific T The cytotoxicity is predominantly mediated granzyme B and, small extent, necrosis factor-α (TNF-α)-related apoptosis-inducing ligand (TRAIL) independent perforin, Fas TNF-α. conclusion, our data provide previously unappreciated differentiation monocytes towards observation capacity lends support implementation protocols.