Somatic embryogenesis of Cyclamen persicum in liquid medium
作者： Marc Kreuger , Erik Postma , Yvon Brouwer , Gerrit-Jan Holst
DOI: 10.1111/J.1399-3054.1995.TB00974.X
关键词: Botany 、 Plantlet 、 Biology 、 Micropropagation 、 Horticulture 、 Explant culture 、 Subculture (biology) 、 Kinetin 、 Cyclamen persicum 、 Somatic embryogenesis 、 Cell culture 、 Plant science 、 Genetics 、 Cell biology 、 Physiology 、 General Medicine
摘要: A method is described for the production of somatic embryos Cyclamen persicum Mill. in liquid medium. Five steps are involved ; initiation embryogenic cell lines, proliferation pro-embryogenic masses (PEMs) on auxin-containing medium, development hormone-free medium with high osmolarity, germination and subsequent plantlet formation. Cell lines were initiated by culturing explant, seedling tuber, directly Three parameters important obtaining explant density, hormone concentrations subculture regime. The rate uptake hormones 2,4-dichlorophenoxyacetic acid (2,4-D) kinetin influenced formation PEMs. Highly obtained only when PEMs had formed within 5-7 weeks. proliferated at least 24 months could be isolated from each embryos. sucrose content (175 mM) without ensured efficient embryo low concentration (58 induced a thus promoting germination. Arabinogalactan-proteins (AGPs) carrot seeds AGPs bound monoclonal antibody ZUM 18 increased number culture, showing that activity not species specific.
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