Characteristics of the α2/β‐adrenoceptor‐coupled adenylate cyclase system and their relationship with adrenergic responsiveness in hamster fat cells from different anatomical sites

摘要: Various studies have shown that the lipolytic response of white adipocytes to catecholamines was dependent on anatomical origin these cells. To provide a biological explanation for this phenomenon, we compared hamster adipocytes, from femoral subcutaneous and epididymal fat, their activities, cAMP responses adrenoceptor-coupled adenylate cyclase system. Basal maximal beta-adrenergic (isoproterenol) mixed alpha 2/beta-adrenergic (epinephrine) agonists were lower in cells than cells, but 2-adrenergic antilipolytic 5-bromo-6-(2-imidazolin-2-ylamino)quinoxaline bi-tartate (UK14304) slightly greater fat Identical results observed responses, except inhibitory which identical both deposits. Adrenoceptors revealed higher density 2-adrenoceptors 2-(2-methoxy-1,4-benzodioxan-2-yl)-2-imidazoline ([3H]RX821002-binding sites) stimulatory beta-adrenoceptors (125I-cyanopindolol-binding similar subdivision into beta-adrenoceptor subtypes adipose Finally, level alpha-subunits inhibitors guanine-nucleotide-binding regulatory proteins, as well catalytic activity 40-50% cell membranes membranes. These suggest differences between result at least part site-related system rather adrenoceptor status.