Enhanced Expression of GM-CSF in Differentiating Eosinophils of Atopic and Atopic Asthmatic Subjects

摘要: Higher numbers of eosinophil/basophil colony-forming units (Eo/B CFU) are observed in blood atopic individuals, and can be enhanced asthmatics by allergen-inhalation challenge. It is known that mature basophils eosinophils synthesize cytokines relevant to allergic inflammation. To investigate the potential role growth factors disease we examined expression hemopoietic cytokines, granulocyte-macrophage colony-stimulating factor (GM-CSF) interleukin (IL)-5, differentiating Eo/B colony cells from normal before after Peripheral was collected two 12 also 25 24 h allergen Nonadherent mononuclear were isolated grown semisolid medium. colonies selected cytospins prepared for immunocytochemical analysis cells. colonies, especially carbol chromotrope 2R+ cells, at Days 10, 14, 18 donors contained messenger RNA GM-CSF combined situ reverse transcription-polymerase chain reaction cytochemistry, demonstrated time-dependent immunocytochemistry (P = 0.007). Atopic individuals a higher percentage expressing than did subjects under all conditions when Day 14 0. 04). challenged with inhaled who dual airway response, an increase number 0.0001), CFU 0.02) significant immunostainable 0009), but only variable effect on those IL-5, allergen. These results suggest Eo/Bs may provide additional stimulus vivo enhance progenitor differentiation asthmatic The concept microenvironmental differentiation, where aid their own supported these ex findings.