Modeling Gliomagenesis with Somatic Cell Gene Transfer using Retroviral Vectors

摘要: Modeling brain tumor formation in experimental animals with somatic cell gene transfer has a long history. In the early experiments naturally occurring retroviruses were used to induce primary tumors variety of test animals. The subsequent identification v-src oncogene within RSV and other cellular proto-oncogenes encoded by moved field oncology into molecular age. Recombinant originally infect cells vitro followed transplantation. Since then, retroviral vectors have been generate glioma vivo via intracerebral injection neonatal mice. most recent models, can be induced injecting avian recombinant containing different oncogenes, individually or combination, transgenic mice genetically engineered for susceptibility targeting specific types brain. Animal modeling contributed substantially understanding etiology leading gliomagenesis. current models provide tumors, which are histologically similar their human counterparts, making them attractive use drug discovery treatment gliomas.