Restoration of wild-type p16 down-regulates vascular endothelial growth factor expression and inhibits angiogenesis in human gliomas.
作者： Yoshiaki Kumon , Saburo Sakaki , Katsuyuki Hamada , Kohji Sato , Kou Nakagawa
DOI:
关键词: Cancer research 、 Neovascularization 、 Vascular endothelial growth factor 、 Angiogenesis 、 Biology 、 Gene product 、 Genetic transfer 、 Genetic enhancement 、 Tumor suppressor gene 、 Glioma
摘要: Recent studies have indicated that the loss of p16 is a frequent event in progression malignant gliomas. The promotes acquisition characteristics gliomas, which are among most angiogenic all human tumors. High-grade gliomas distinguished from low-grade by intense angiogenesis addition to their p16. New therapeutic strategies aimed at inhibiting tumor on basis molecular mechanisms theoretically attractive. Here we evaluate effect gene replacement Infection with recombinant replication-defective adenovirus vector containing cDNA wild-type significantly reduced expression vascular endothelial growth factor, thought be pivotal mediator angiogenesis, p16-deleted glioma cells. Restoring into cells markedly inhibited induced vivo. Furthermore, neovascularization more potently than did p53 transfer. These findings indicate plays an important role regulation suggesting novel function gene.
aacrjournals.org参考文章(33)
Maglione D, Califano D, Chiappetta G, Santelli G, Mineo A, Monaco C, Lago Ct, Botti G, Viglietto G, Romano A, Rambaldi M, Paoletti I, Persico Mg, Manzo G, Neovascularization in human germ cell tumors correlates with a marked increase in the expression of the vascular endothelial growth factor but not the placenta-derived growth factor. Oncogene. ,vol. 13, pp. 577- 587 ,(1996)
G Reifenberger, K Ichimura, V P Collins, E E Schmidt, CDKN2 (p16/MTS1) gene deletion or CDK4 amplification occurs in the majority of glioblastomas. Cancer Research. ,vol. 54, pp. 6321- 6324 ,(1994)
Jin Jen, J Wade Harper, Sandra H Bigner, Darell D Bigner, Nickolas Papadopoulos, Sanford Markowitz, James KV Willson, Kenneth W Kinzler, Bert Vogelstein, None, Deletion of p16 and p15 Genes in Brain Tumors Cancer Research. ,vol. 54, pp. 6353- 6358 ,(1994)
Lucien Jules Rubinstein, Dorothy S. Russell, Pathology of tumours of the nervous system ,(1977)
Olga V. Volpert, Peter J. Polverini, Noël P. Bouck, Pauline Chou, Peter A. Steck, Sambasiva Rao, Stephanie C. Hsu, Tom Mikkelsen, Inhibition of Angiogenesis in Human Glioblastomas by Chromosome 10 Induction of Thrombospondin-1 Cancer Research. ,vol. 56, pp. 5684- 5691 ,(1996)
Thomas Boehm, Judah Folkman, Timothy Browder, Michael S. O'Reilly, Antiangiogenic therapy of experimental cancer does not induce acquired drug resistance Nature. ,vol. 390, pp. 404- 407 ,(1997) , 10.1038/37126
Candelaria Gomez-Manzano, W. K. Alfred Yung, Kim D. Steck, Jack A. Roth, Juan Fueyo, Victor A. Levin, Peter A. Steck, Timothy J. McDonnell, Athanassios P. Kyritsis, Adenovirus-mediated transfer of the p53 gene produces rapid and generalized death of human glioma cells via apoptosis. Cancer Research. ,vol. 56, pp. 694- 699 ,(1996)
Mike Bradburn, Tim O'Brien, Sue Fuggle, Jeremy P. Crew, Roy Bicknell, David Cranston, Adrian L. Harris, Vascular endothelial growth factor is a predictor of relapse and stage progression in superficial bladder cancer. Cancer Research. ,vol. 57, pp. 5281- 5285 ,(1997)
Leonidas Tsiokas, Vikas P. Sukhatme, Debabrata Mukhopadhyay, Wild-Type p53 and v-Src Exert Opposing Influences on Human Vascular Endothelial Growth Factor Gene Expression Cancer Research. ,vol. 55, pp. 6161- 6165 ,(1995)
Kazumoto Iijima, Norishige Yoshikawa, Hajime Nakamura, Activation-induced expression of vascular permeability factor by human peripheral T cells: a non-radioisotopic semiquantitative reverse transcription-polymerase chain reaction assay Journal of Immunological Methods. ,vol. 196, pp. 199- 209 ,(1996) , 10.1016/0022-1759(96)00129-9