Cell lineage segregation during bursa of Fabricius ontogeny.

摘要: The population dynamics of myeloid and lymphoid lineages during bursa Fabricius ontogeny were analyzed by immunofluorescence using two monoclonal antibodies (mAb). CL-1 mAb reacts with all chicken hemopoietic cells, except mature erythrocytes. L22 bursa-derived lymphocytes, a minor subset macrophages some cells the thymic medulla. staining embryonic bursas these helps to distinguish between different cells: CL-1+/L22+ represent B lymphocytes macrophages, while CL-1+/L22- correspond most granulocytes, which disappear at end life. as well first observed outside bursal rudiment. This indicates that there is pre-bursal segregation kinds precursors colonize rudiment about same time (day 9 for days or 10 cells). Moreover our data show colonization epithelium two-step phenomenon. enter belong lineage, express Ia-like antigens high level, are dendritic in morphology, macrophage/dendritic cell lineage. They responsible formation epithelial bud then colonized small number Quail-chick grafting experiments also performed grafts examined (restricted cells) reactivity. These observations confirmed previous findings kinetics give support pathways.