The role of sexual steroid hormones in the direct stimulation by Kisspeptin-10 of the secretion of luteinizing hormone, follicle-stimulating hormone and prolactin from bovine anterior pituitary cells

作者: A. Ahmed Ezzat , H. Saito , T. Sawada , T. Yaegashi , Y. Goto

DOI: 10.1016/J.ANIREPROSCI.2010.06.002

关键词: Follicle-stimulating hormoneGonadotropic cellLuteinizing hormoneHormoneTestosteroneProlactinChemistryAnterior pituitaryEndocrinologyKisspeptinInternal medicine

摘要: Abstract The aims of the present study were to clarify effect Kisspeptin-10 (Kp10) on secretion luteinizing hormone (LH), follicle-stimulating (FSH) and prolactin (PRL) from bovine anterior pituitary (AP) cells evaluate ability sex steroids enhance sensitivity gonadotropic lactotropic Kp10. AP prepared 7-week-old male calves incubated for 12 h with estradiol (E2; 10−8 M), progesterone (P4; testosterone (T; or vehicle only (control), then 2 h Kp10 (10−6 M). amounts LH, FSH PRL released into culture medium after 2-h incubation period examined. significantly stimulated LH treated E2 T (P  results suggest that T, but not P4, in response However, had no stimulatory regardless steroids. directly stimulates cells, do Furthermore, LH- FSH-releasing PRL-releasing are less potent than GnRH TRH, respectively.

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