Growth Regulation of Human Breast and Ovarian Tumor Cells by Heregulin: Evidence for the Requirement of ErbB2 as a Critical Component in Mediating Heregulin Responsiveness

摘要: Alterations in the expression of epidermal growth factor (EGF) receptor ErbB family are frequently encountered a number human cancers. Two these receptors, ErbB3 and ErbB4, known to bind related proteins termed heregulins (HRGs) or neu differentiation factors. In biologically relevant systems, interaction HRG with ErbB4 results transactivation ErbB2. this report, we show that ErbB2 is critical component mediating responsiveness panel breast ovarian tumor cell lines. Because HRGs have been reported elicit diverse biological effects on cultured cells, including stimulation, inhibition, induction differentiation, systematically examined effect rHRG beta 1 proliferation. binding studies were performed lines expressing range levels The responses also compared EGF growth-inhibitory anti-ErbB2 antibody, 4D5. most cases, stimulation DNA synthesis correlated positive cycle progression enhancement colony formation soft agar. On each line tested, distinguishable from Our findings indicate induces proliferation addition, methods for measuring proliferation, as well experimental conditions, determining vitro.