Pyruvate kinase in malaria host–parasite interaction

摘要: THE mammalian malaria host–parasite systems seem particularly useful for studies aimed at elucidating the biochemical mechanisms of such interactions. During vertebrate phase their life cycles organisms are intracellular parasites red cell. The mature host cell is also relatively simple metabolically; its energy metabolism solely that Embden–Meyerhof glycolysis and it lacks capacity protein synthesis. We therefore studied several aspects in monkeys heavily infected with Plasmodium knowlesi mice P. berghei. In both these we have found an increase blood adenosine triphosphate (ATP) a decrease 2,3-diphosphoglycerate (DPG). evidence introduce pyruvate kinase isozyme into cells amounts sufficient to alter glycolysis. First, glycolytic intermediate data demonstrate vivo activity cells; second, there Vmax third, gel electrophoretic patterns show new cells. suggest this alteration direction favourable parasite because will ATP expense DPG.