Genetic polymorphisms of cytochrome p450 2E1, glutathione S-transferase M1 and T1, and susceptibility to gastric carcinoma in Taiwan.

摘要: Background and aims: Cytochrome P450 (CYP) glutathione S-transferase (GST) enzymes are involved in activation detoxification of many potential carcinogens. Genetic polymorphisms these have been found to influence interindividual interethnic susceptibility cancer. Although CYP GST the N-nitrosamines related compound, studies on relationship between genetic CYP2E1, GSTT1, GSTM1 risk gastric carcinoma (GC) few, results conflicting. Patients methods: We conducted a hospital-based case-control study investigate whether such variations affect developing GC. Subjects included 356 GC patients 278 unaffected controls. Peripheral white blood cell DNA was obtained from all subjects. Genotyping CYP2E1 performed using PCR-based restriction fragment length polymorphism assay. Deletion GSTT1 genes assessed by multiplex PCR. Results: The distribution c2/c2 genotype detected PstI or RsaI digestion, differed significantly controls; odds ratio 2.9. It remained significant after adjustment with gender, histological subtypes (diffuse, intestinal, mixed), location (cardia, body, antrum/angle), stage (early, advanced). In contrast, prevalence DraI null similar controls patients. Conclusion: Our findings suggest that is determinant Taiwan.