High resolution melting analysis for rapid and sensitive EGFR and KRAS mutation detection in formalin fixed paraffin embedded biopsies
作者： Hongdo Do , Michael Krypuy , Paul L Mitchell , Stephen B Fox , Alexander Dobrovic
关键词: Gefitinib 、 Epidermal growth factor receptor 、 High Resolution Melt 、 Adenocarcinoma 、 Biology 、 EGFR inhibitors 、 Mutation (genetic algorithm) 、 Growth factor receptor 、 Epidermal growth factor 、 Molecular biology
摘要: Background Epithelial growth factor receptor (EGFR) and KRAS mutation status have been reported as predictive markers of tumour response to EGFR inhibitors. High resolution melting (HRM) analysis is an attractive screening method for the detection both known unknown mutations it rapid set up inexpensive operate. However, now has not fully validated clinical samples when formalin-fixed paraffin-embedded (FFPE) sections are only material available often case.
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