Nerve growth factor-mediated induction of tyrosine hydroxylase in rat superior cervical ganglia in vitro.
作者： H Thoenen , H Rohrer , S R Max , U Otten
DOI: 10.1016/S0021-9258(17)34353-3
关键词: Nerve growth factor 、 Tyrosine 、 Tyrosine hydroxylase 、 Organ culture 、 In vitro 、 Endocrinology 、 In vivo 、 Biology 、 Leucine 、 Internal medicine 、 Cervical ganglia
摘要: Exposure of rat sympathetic ganglia to 3 microgram/ml 2.5 S nerve growth factor (NGF) resulted in a 100% increase tyrosine hydroxylase activity within 48 h. Pulselabeling proteins with [3H]leucine, followed by immunoprecipitation antibodies hydorxylase and isolation the precipitated enzyme gel electrophoresis, demonstrated that was due enhanced de novo synthesis. The incorporation [3H]leucine into increased 150% compared 17% total protein synthesis, which not statistically significant. fact half-life pulse-labeled same for NGF-treated control organ cultures superior cervical excludes possibility labeling NGF is decreased degradation. We conclude that, without modulatory factors play role vivo, can enhance synthesis vitro, provided culture conditions permit optimal survival adrenergic neurons are selected.
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