Cultural Studies of Protoplasts and Leaf Callus of Trigonella corniculata and T. foenum-graecum
作者： A.V.P. dos Santos , M.R. Davey , E.C. Cocking
DOI: 10.1016/S0044-328X(83)80224-4
关键词: Trigonella corniculata 、 Shoot 、 Biology 、 Explant culture 、 Botany 、 Callus 、 Plating efficiency 、 Horticulture 、 Protoplast 、 Liquid medium 、 Environment controlled 、 General Medicine
摘要: Summary Mesophyll protoplasts were isolated using a Biogel-purified enzyme mixture from leaflets of vegetative and flowering stems Trigonella corniculata T. foenum-graecum grown in controlled environment cabinet. Protoplasts both species divided liquid medium with plating efficiency 70%. Cell suspensions initiated leaf callus yielded which formed callus. In contrast, not obtained cell . Callus derived explants, mesophyll suspension embryoids shoots, but tissues recalcitrant to regeneration. These results, including the difficulties associated , are discussed relation recent progress on regeneration forage legumes.
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