K-ras oncogene activation in adenocarcinoma of the human pancreas. A study of 82 carcinomas using a combination of mutant-enriched polymerase chain reaction analysis and allele-specific oligonucleotide hybridization.

摘要: We examined 82 surgically resected or biopsied, formalin-fixed, paraffin-embedded primary adenocarcinomas of the pancreas for presence activating point mutations in codon 12 K-ras oncogene. Mutations were detected using primer-mediated, mutant-enriched, polymerase chain reaction-restriction fragment length polymorphism analysis and characterized further by allele-specific oligonucleotide hybridization. This combination mutant-enriched hybridization results a rapid sensitive characterization K-ras. Sixty-eight (83%) carcinomas harbored mutation. Of 68 mutations, 33 (49%) guanine to adenine transitions, 27 (39%) thymine transversions, eight (12%) cytosine transversions. found head (61 75, 81%) as well body tail (seven seven, 100%) pancreas. The overall prevalence obtained from patients who smoked cigarettes at some during their lives (88%; 86% current smokers 89% ex-smokers) was greater than that seen pancreatic never (68%, P = 0.046). did not correlate with tumor ploidy, proliferating index, patient survival. These demonstrate combined can be used detect characterize oncogene tissues, confirm occur frequently