作者: Abujiang Pataer , Kazumi Yoshida , Yanna Liu , Xiaolin Lu , Stephen G. Swisher
关键词: Transfection 、 Transcription factor 、 Cancer cell 、 E2F 、 Apoptosis 、 Caspase 、 Apoptosis-inducing factor 、 Molecular biology 、 Downregulation and upregulation 、 Biology
摘要: Background: Overexpression of the transcription factor E2F-1 provokes apoptosis in cancer cells; mechanism, however, is not completely understood. We sought to evaluate gene therapy human colon and investigate apoptotic pathway involved. Methods: Adenoviral vectors were used transfect (Ad5E2F-1) or control luciferase (Ad5Luc) into four carcinoma cell lines. Apoptosis was confirmed by flow cytometry poly (ADP-ribose) polymerase cleavage. Expression factors determined with Western blot analysis. Inhibitory assays determine involvement caspases pathway. Results: evident all cells treated Ad5E2F-1; upregulation Bcl-2, activation noted. The apoptosis-inducing cytosolic fraction markedly upregulated after Ad5E2F-1 treatment. overexpression inhibited proliferation induced significant lines (P < .005). This response could be only partially blocked caspase inhibitors. Conclusions: These findings demonstrate that induces inhibits marked fact E2F-1–induced incompletely inhibitors suggest a caspase-independent E2F-1–mediated apoptosis, reported here for first time.