Sex-sorting of spermatozoa affects developmental competence of in vitro fertilized oocytes in a bull-dependent manner
作者： Yasushi INABA , Reika ABE , Masaya GESHI , Satoko MATOBA , Takashi NAGAI
DOI: 10.1262/JRD.2016-032
关键词: Andrology 、 Human fertilization 、 Sperm 、 Blastocyst 、 Oocyte 、 Dependent manner 、 In vitro fertilisation 、 Biology 、 Embryogenesis 、 In vitro
摘要: The aim of the present study was to clarify if flow-cytometric sex-sorting bovine sperm affected in vitro blastocyst production different bulls, either terms its ability fertilize oocyte or by interfering with post-fertilization embryo development. We performed fertilization (IVF) using both commercially available frozen-thawed X-sorted and non-sorted 4 Holstein bulls at 3 concentrations (1 × 106, 2 5 106 sperm/ml). When rates were compared, a variation among detected similar results for sperm. However, we found no evidence that sorting process. To investigate effects on development, determined optimum concentration IVF each bull, which resulted bulls. next their compared Cleavage counterparts. significantly reduced development associated use one whereas other three after similar. In conclusion, our system, X-sorting affects reducing developmental competence fertilized oocytes rather than affecting occurrence this phenomenon varies
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