Optimized in vitro production of Plasmodium vivax ookinetes.
作者： Colleen M. McClean , Victor Neyra , Joseph M. Vinetz , Haydee Guerra Alvarado , Alejandro Llanos-Cuentas
DOI: 10.4269/AJTMH.2010.10-0195
关键词: Xanthurenic acid 、 Plasmodium vivax 、 Biology 、 Gametocyte 、 Tris 、 Fetal bovine serum 、 Gametogenesis 、 Hypoxanthine 、 HEPES 、 Andrology 、 Virology
摘要: Previous reports have described obtaining mature Plasmodium vivax ookinetes in vitro using blood from infected patients a simplified, field-based protocol. Here, we report protocols that produce improved P. ooki- nete yields and morphological development. Optimal conditions included induction of gametogenesis 10 mM Tris, 170 NaCl, glucose, 25 NaHCO 3 , 100 μM xanthurenic acid for 90 minutes at pH 8.0-8.2, followed by culture RPMI-1640, 50 mg/mL hypoxanthine, HEPES, 29 2 L-glutamine, 20% fetal bovine serum 8.4 36 hours. Ookinetes were produced 86% (18/21) optimized cultures; ranged 6.5 × 4 to 2.8 6 ; percent gametocyte conversion 1.4% 4.7%. This method is suitable preparation quantities sufficient biochemical, molecular, cell biological analysis where basic laboratory facilities are proximity with malaria.
cabdirect.org
ajtmh.org参考文章(22)
Gabriela E Zollner, Narong Ponsa, Gabriel W Garman, Shreekanta Poudel, Jeffrey A Bell, Jetsumon Sattabongkot, Russell E Coleman, Jefferson A Vaughan, Population dynamics of sporogony for Plasmodium vivax parasites from western Thailand developing within three species of colonized Anopheles mosquitoes. Malaria Journal. ,vol. 5, pp. 68- 68 ,(2006) , 10.1186/1475-2875-5-68
E. H. Carter, A. Suhrbier, P. J. A. Beckers, R. E. Sinden, The in vitro cultivation of P. falciparum ookinetes, and their enrichment on Nycodenz® density gradients Parasitology. ,vol. 95, pp. 25- 30 ,(1987) , 10.1017/S0031182000057516
Hilary Hurd, Ebtesam Al-Olayan, Geoffrey A. Butcher, In vitro methods for culturing vertebrate and mosquito stages of Plasmodium. Microbes and Infection. ,vol. 5, pp. 321- 327 ,(2003) , 10.1016/S1286-4579(03)00034-0
R.E. Sinden, Sexual development of malarial parasites in their mosquito vectors. Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 75, pp. 171- 172 ,(1981) , 10.1016/0035-9203(81)90058-4
David C. Kaslow, Transmission-blocking vaccines: uses and current status of development. International Journal for Parasitology. ,vol. 27, pp. 183- 189 ,(1997) , 10.1016/S0020-7519(96)00148-8
Shreekanta S. Poudel, Robert A. Newman, Jefferson A. Vaughan, Rodent Plasmodium: Population Dynamics of Early Sporogony within Anopheles stephensi Mosquitoes Journal of Parasitology. ,vol. 94, pp. 999- 1008 ,(2008) , 10.1645/GE-1407.1
M. P. Weinstein, D. V. Alcid, Occurrence of exflagellation and microgametes in peripheral blood of a patient with malaria The Journal of Infectious Diseases. ,vol. 146, pp. 448- 448 ,(1982) , 10.1093/INFDIS/146.3.448
Kailash P. Patra, Jeff R. Johnson, Greg T. Cantin, John R. Yates, Joseph M. Vinetz, Proteomic analysis of zygote and ookinete stages of the avian malaria parasite Plasmodium gallinaceum delineates the homologous proteomes of the lethal human malaria parasite Plasmodium falciparum Proteomics. ,vol. 8, pp. 2492- 2499 ,(2008) , 10.1002/PMIC.200700727
A. Knight, R.E. Sinden, The purification of gametocytes of Plasmodium falciparum and P. yoelii nigeriensis by colloidal silica (Percoll) gradient centrifugation Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 76, pp. 503- 509 ,(1982) , 10.1016/0035-9203(82)90150-X
Rachanee Udomsangpetch, Osamu Kaneko, Kesinee Chotivanich, Jetsumon Sattabongkot, Cultivation of Plasmodium vivax. Trends in Parasitology. ,vol. 24, pp. 85- 88 ,(2008) , 10.1016/J.PT.2007.09.010