The influence of cell elastic modulus on inertial positions in Poiseuille microflows.

摘要: Abstract Microchannels are used as a transportation highway for suspended cells both in vivo and ex vivo. Lymphatic cardiovascular systems transfer through microchannels within the body, microfluidic techniques such lab-on-a-chip devices, flow cytometry, CAR T-cell therapy utilize of similar sizes to analyze or separate Understanding forces that subject while traveling these channels important because certain applications exploit cell properties separation. This study investigated influence cytoskeletal impairment has on inertial positions circulating in laminar pipe flow. Two representative cancer lines were treated using cytochalasin D, their particle streak imaging compared between benign metastatic lines. resulted shift well untreated cells. To determine quantify physical changes this migration, staining nanoindentation then cells’ size, circularity, elastic modulus. It was found exposure D decreased moduli cells, with showing decreases 135 ± 91 130 60 Pa, respectively, no change either size shape. caused benign, stiffer be more evenly distributed across channel width than metastatic, deformable cells; additionally, decrease increased migration toward center. These results indicate modulus may play part previously thought.