Sequence variation in the promoter region of the cholinergic receptor muscarinic 3 gene and asthma and atopy

摘要: Abstract Background: Muscarinic acetylcholine receptors are members of the superfamily G protein–coupled, 7 transmembrane– spanning proteins. They important in development airway hyperresponsiveness. In lung M 3 receptor, encoded by cholinergic receptor muscarinic gene, is present smooth muscle and mediates contraction. Objective: We considered gene as a possible candidate for bronchial asthma initiated studies to identify polymorphisms promoter region. Method: identified 4 single-nucleotide (−708A/G, −627G/C, −513C/A, −492C/T) 2 short tandem repeat polymorphisms, tetranucleotide (CTTT) 12-20 dinucleotide (GT) 6-19 repeat. Results: None single nucleotide were significantly more frequent asthmatic patients (n = 76) compared with healthy control subjects 81). Furthermore, there was no evidence nonrandom transmission polymorphism haplotypes individuals or hyperresponsiveness ( P > .50) large Hutterite pedigree. However, significant skin test reactivity cockroach allergens (global disequilibrium test: χ 38.55, .013). Conclusions: These results suggest role this atopic disorders. (J Allergy Clin Immunol 2003;111:527-32.)