Genome-wide Association Study Using Pooled DNA to Identify Candidate Markers Mediating Susceptibility to Postoperative Nausea and Vomiting
作者： Piotr K. Janicki , Ryan Vealey , Jiabin Liu , Jeremiah Escajeda , Marek Postula
DOI: 10.1097/ALN.0B013E31821810C7
关键词:
摘要: Background: A family history has been established as a risk factor for postoperative nausea and vomiting (PONV), but the identities of susceptibility genes remain unknown. The goal this study was to identify genetic loci that may contribute PONV in an adult population. Methods: authors performed genome-wide association involving pooling DNA obtained from 122 patients with severe 129 matched controls. Each pool hybridized single nucleotide polymorphism (SNP) microarray, probe intensity used predict allele frequency. Differences frequency between SNP control groups were ranked after accounting error. highest ranking SNPs selected individual genotyping subjects whom comprised new verification cohort consisting 208 (104 104 controls). Results: identified 41 targets showing substantial difference allelic pools. These markers first genotyped samples which pools comprised. observed evidence 19 different genome. In separate cohort, four SNPs. This remained significant correcting multiple testing (P < 0.0023) one (rs2165870), is located upstream promoter muscarinic acetylcholine receptor 3 subtype (CHRM3) gene. Conclusions: using pooled samples. Through genotyping, they confirmed at least predictive susceptibility.
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