Immunological response to tissue-engineered cartilage derived from auricular chondrocytes and a PLLA scaffold in transgenic mice.
作者： Yuko Fujihara , Tsuyoshi Takato , Kazuto Hoshi
DOI: 10.1016/J.BIOMATERIALS.2009.10.053
关键词: Transgene 、 Cartilage transplantation 、 Genetically modified mouse 、 Cartilage 、 Cell biology 、 Immune system 、 Immunology 、 Immune privilege 、 Chemistry 、 Macrophage migration inhibitory factor 、 Fas ligand
摘要: The immune response against biomaterials in tissue-engineered constructs could potentially worsen the outcome of tissue regeneration, but immunological reactions between host and donor remain to be clarified. In present study, we syngenically transplanted cartilage consisting C57BL/6 mice auricular chondrocytes poly-l-lactic acid scaffolds (MW:200,000) into EGFP transgenic background, evaluated by localization donor-derived host-derived cells, latter which were distinguished presence EGFP. While cells constituted areas regenerated cartilage, increased number for initial two weeks, then decreased excluded non-cartilage thereafter. Furthermore, positivity was mostly co-localized with that F4/80, suggesting most macrophages. Immunohistochemical staining revealed expression factors related privilege chondrocytes, such as macrophage migration inhibitory factor (MIF), fas ligand (FasL) others. Co-culture macrophages vitro MIF FasL regulate actions expressing privilege.
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