Melanocyte-specific CD8+ T cells are associated with epidermal depigmentation in a novel mouse model of vitiligo

摘要: In the present study, we established a novel murine model of vitiligo by sequential prime/boost immunizations into hind footpad and tail dermis with tyrosinase-related protein 2 (TRP2)-180 (SVYDFFVWL) peptide, lipopolysaccharides cytosine-phosphate-guanosine (CpG) oligodeoxynucleotides. Immunized mice developed epidermal depigmentation in skin without hair depigmentation, thereby differentiating this approach from models vitiligo. Following intradermal immunization, activated CD8(+) interferon (IFN)-γ(+) T cells were recruited locally to skin. In-vivo cytotoxicity assays demonstrated specific lysis TRP2-180-presenting immunized mice. Furthermore, extent correlated frequency TRP2-180-specific splenic cells, as determined IFN-γ tumour necrosis factor (TNF)-α production, cytotoxic degranulation evidenced CD107a staining. These findings suggest correlation between presence effector development depigmented lesions our model. This new vitiligo, characterized is more similar human disease than previous models. Therefore, well suited future studies on pathogenesis therapeutics for