Reduction of nifurtimox and nitrofurantoin to free radical metabolites by rat liver mitochondria. Evidence of an outer membrane-located nitroreductase.
作者： S N Moreno , R P Mason , R Docampo
DOI: 10.1016/S0021-9258(20)82140-1
关键词: Nitroreductase 、 Enzyme 、 Nifurtimox 、 NAD+ kinase 、 Superoxide 、 Biochemistry 、 Chemistry 、 Bacterial outer membrane 、 Mitochondrion 、 Respiratory chain
摘要: Nifurtimox and nitrofurantoin are reduced by intact rat liver mitochondria to nitro anion radicals whose autoxidation generates superoxide as detected direct electron spin resonance spectroscopy spin-trapping experiments, respectively. Although nitroreduction occurred in the presence of respiratory substrates such beta-hydroxybutyrate, malate-glutamate, succinate, or endogenous substrates, radical formation activity was much greater on addition exogenous pyridine nucleotides. NAD(P)H generated from mitochondrial NAD(P)+ intramitochondrial reactions could not be used for nitroreductase unless were solubilized detergent. In addition, crude outer membrane fraction, with a higher than mitoplasts mitochondria. These results provide evidence nitrofuran reductase associated that is far more important chain enzymes.
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