Hydralazine-dependent carbon dioxide free radical formation by metabolizing mitochondria.
作者： P K Wong , J L Poyer , C M DuBose , R A Floyd
DOI: 10.1016/S0021-9258(18)37957-2
关键词:
摘要: The addition of hydralazine (1-hydrazinophthalazine) to rat liver mitochondria metabolizing malate/glutamate causes formation a carbon-centered free radical which was spin-trapped with phenyl-t-butylnitrone (PBN) or dimethylpyrrolidine-N-oxide (DMPO). coupling constants the were AN = 16.1, AH beta 4.6 G for PBN and 15.9, 18.9 DMPO-trapped in aqueous solution. shown be carbon dioxide anion by independent synthesis, high pressure liquid chromatography separation, electron paramagnetic resonance characterization. amount produced absolutely dependent upon presence varied depending on mitochondrial substrate, far highest pyruvate. Studies 13C-labeled pyruvate demonstrated that came from C-1 this compound.
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