Refined deletion mapping of the chromosome 19q glioma tumor suppressor gene to the D19S412-STD interval.

摘要: Allelic loss of chromsome 19q occurs frequently in malignant gliomas, suggesting the presence a chromosome glioma tumor suppressor gene. Deletion mapping studies have delineated 3.5 Mb candidate region between D19S219 and HRC. Cloned sequences from proximal 425 kb this interval, however, not shown tumor-specific alterations. To refine location gene further, we conducted heterozygosity on 191 gliomas using nine PCR-based polymorphisms. These included previously identified physically mapped markers D19S219, DM, D19S112, HRC recently polymorphisms at D19S412, STD, D19S596 GYS. In addition, isolated novel microsatellite polymorphism that maps 400 telomeric to D19S112. Oligodendroglial tumors showed frequent all grades, typically displayed allelic studied markers. Astrocytomas, primarily anaplastic astrocytomas deletion breakpoints within region. revealed minimal overlap D19S412 distance 900 kb. data suggest D19S412-STD interval represents most likely for involved astrocytoma, perhaps oligodendroglioma, tumorigenesis.