Electric Conductivity and Resistance of Mouse Oocyte Membranes to Effect of Pulsed Electric Field in Cryoprotectant Solutions
作者： Yevgeniya I. Smolyaninova , , Viktor O. Shigimaga , Alla O. Kolesnikova , Ludmyla I. Popivnenko
关键词: Conductometry 、 Analytical chemistry 、 Cryoprotectant 、 Plasma 、 Ethylene glycol 、 Chemistry 、 Electrical resistivity and conductivity 、 Membrane 、 Dimethyl sulfoxide 、 Electric field
摘要: Using the method of pulsed conductometry, values electric conductivity mouse oocytes in 1.0 M solutions penetrating cryoprotectants have been first determined and stability their plasma membranes to effect field increasing intensity has investigated. For oocytes, incubated ethylene glycol (EG), 1,2-propanediol (1,2-PD), 2,3-butanediol (2,3-BD) dimethyl sulfoxide (DMSO) solutions, were (23.6 ± 3.4), (21.3 5.8) (21.0 2.3) 7.1) μS/cm respectively. The for acetamide (AC) formamide (FA) (16.5 6.1) (16.9 ±± 10.7) μS/cm, Cryoprotectant belonging alcohols (EG, 2,3-BD), as well DMSO, shown a stabilizing on oocyte under action. AC FA caused no membranes, which was manifested development irreversible breakdown with intensity. Probl Cryobiol Cryomed 2018; 28(4): 311-321
sci-hub.st 参考文章(18)
S Souchelnytskyi, None, Individualization of cancer treatment: contribution of OMICs technologies to cancer diagnostic Biotechnologia Acta. ,vol. 6, pp. 105- 117 ,(2013) , 10.15407/BIOTECH6.04.105
Leonid V. Chernomordik, 5 – Electropores in Lipid Bilayers and Cell Membranes Guide to Electroporation and Electrofusion. pp. 63- 76 ,(1991) , 10.1016/B978-0-08-091727-6.50008-3
Benjamin P. Best, Cryoprotectant Toxicity: Facts, Issues, and Questions Rejuvenation Research. ,vol. 18, pp. 422- 436 ,(2015) , 10.1089/REJ.2014.1656
V. A. Shigimaga, Pulsed conductometer for biological cells and liquid media Measurement Techniques. ,vol. 55, pp. 1294- 1300 ,(2013) , 10.1007/S11018-013-0124-2
Gregory M. Fahy, The relevance of cryoprotectant "toxicity" to cryobiology. Cryobiology. ,vol. 23, pp. 1- 13 ,(1985) , 10.1016/0011-2240(86)90013-1
M. Laura Fernández, Ramon Reigada, Effects of dimethyl sulfoxide on lipid membrane electroporation. Journal of Physical Chemistry B. ,vol. 118, pp. 9306- 9312 ,(2014) , 10.1021/JP503502S
James C. Weaver, Yu.A. Chizmadzhev, Theory of electroporation: A review Bioelectrochemistry and Bioenergetics. ,vol. 41, pp. 135- 160 ,(1996) , 10.1016/S0302-4598(96)05062-3
U. Zimmermann, Electric field-mediated fusion and related electrical phenomena Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes. ,vol. 694, pp. 227- 277 ,(1982) , 10.1016/0304-4157(82)90007-7
Marie-Pierre Rols, Electropermeabilization, a physical method for the delivery of therapeutic molecules into cells Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 1758, pp. 423- 428 ,(2006) , 10.1016/J.BBAMEM.2006.01.005
John H. Crowe, Folkert A. Hoekstra, Lois M. Crowe, Thomas J. Anchordoguy, Erma Drobnis, Lipid phase transitions measured in intact cells with fourier transform infrared spectroscopy Cryobiology. ,vol. 26, pp. 76- 84 ,(1989) , 10.1016/0011-2240(89)90035-7