Comparative proteome analysis of the capsule from patients with frozen shoulder.

摘要: Background The etiology of frozen shoulder (FS) is unclear. Accordingly, this study used a label-free quantitative shotgun proteomic approach to elucidate the pathogenesis FS based on protein expression levels. Methods Tissue samples from rotator interval (RI), middle glenohumeral ligament (MGHL), and anterior-inferior (IGHL) were collected 12 FSs with severe stiffness 7 shoulders cuff tear (RCT) as controls. Protein mixtures digested analyzed by nano-liquid chromatography/electrospray ionization–tandem mass spectrometry. Relative levels calculated signal intensity identified peptide ions spectra. Differentially expressed proteins between RCT evaluated gene enrichment analysis using Gene Ontology Kyoto Encyclopedia Genes Genomes. Results We 1594 proteins, 1358 which in all 6 tissue groups. detected more upregulated upper (RI MGHL) groups lower group than comparative groups, respectively. Various functions repair, collagen metabolism fibrillation, cell–cell cell–matrix adhesion, blood coagulation, immune response highly RI MGHL group. Proteins phagocytosis, glutathione metabolism, retinoid cholesterol IGHL Conclusions pathophysiology differs parts joint capsule. Different treatment strategies for may be appropriate, depending location.