Validation and application of a canine-specific automated high-sensitivity C-reactive protein assay.

摘要: Measurement of low concentrations C-reactive protein (CRP) in dogs has previously been performed with nonautomated assays. The aim this study was to validate an automated high-sensitivity CRP (hsCRP) assay, developed by modifying a routinely used canine-specific immunoturbidimetric test (cCRP). Imprecision, linearity under dilution, limit blank (LOB), detection (LOD), and quantification (LOQ) were determined for the hsCRP test, as well presence prozone effect interferences. imprecision, measured intra-assay variation, ≤2.7%. assay acceptably linear dilution. An analytically relevant present samples concentration >150 mg/L, there mild interferences from hemolysis lipemia. LOB, LOD, LOQ 0.10 0.22 0.50 respectively. A method comparison enzyme-linked immunosorbent (ELISA) performed, showing poor agreement between ELISA. additional apply clinical research samples. Serum 7 undergoing ovariohysterectomy collected pre- postoperatively, both cCRP assay. expected postoperative increase detected earlier compared test. further applied on 6 lean 9 overweight dogs. There no significant difference groups (P = 0.06). In conclusion, had acceptable analytical performance, successfully