Physiological Concentrations of Retinoic Acid Favor Myeloid Dendritic Cell Development over Granulocyte Development in Cultures of Bone Marrow Cells from Mice

摘要: Differentiation of hematopoietic progenitors to dendritic cells (DCs) is a complex, poorly understood process regulated by cytokines, colony-stimulating factors, growth factor receptors, and transcription factors. However, nutritional factors may play an important role. Vitamin A essential for proper immune function implicated in the development myeloid lineage cells, especially granulocytes. We investigated role vitamin differentiation DCs. Cultures bone marrow from mice stimulated with granulocyte-macrophage (GM-CSF) medium reduced serum retinol demonstrated significantly decreased DC compared control cultures containing retinol. Surprisingly, granulocytes predominated GM-CSF when was depleted. The addition all-trans or 9-cis retinoic acid depleted restored DCs inhibited granulocyte development. DC-promoting effect specific because flt-3 ligand. also affected major histocompatibility complex (MHC) class II costimulatory molecule expression. In response increasing concentrations A, expression MHC on DC, whereas molecules increased, CD86. Our data suggest that favors immature instead dietary adequate, deficiency compromise adaptive responses depend antigen presentation.