Plant-originated glycoprotein (24 kDa) has an inhibitory effect on proliferation of BNL CL.2 cells in response to di(2-ethylhexyl)phthalate.

摘要: Di(2-ethylhexyl)phthalate (DEHP) is one of the many environmental chemicals that are widely used in polyvinyl chloride products, vinyl flooring, food packaging and infant toys. They cause cell proliferation or dysfunction human liver. The purpose this study to investigate inhibitory effect a glycoprotein (24 kDa) isolated from Zanthoxylum piperitum DC (ZPDC) on liver DEHP-induced BNL CL. 2 cells. [3H]-thymidine incorporation, intracellular reactive oxygen species (ROS), Ca2+ mobilization activity protein kinase C (PKC) were measured using radioactivity fluorescence method respectively. expression mitogen-activated kinases [extracellular signal-regulated (ERK) c-Jun N-terminal (JNK)], activator (AP)-1 (c-Jun c-Fos), proliferating nuclear antigen (PCNA) cycle-related factors (cyclin D1/cyclin-dependent [CDK] 4) evaluated Western blotting electrophoretic mobility shift assay. results showed levels ROS, PKCα inhibited by ZPDC (100 µg/ml) Also, activities ERK, JNK AP-1 reduced (100 µg/ml). With regard proliferation, PCNA cyclin D1/CDK4 significantly suppressed at treatment with presence DEHP. Taken together, these findings suggest normalized D1/CDK4, which relate factors. Thus, appears be compounds derived natural products able inhibit phthalate-induced Copyright © 2011 John Wiley & Sons, Ltd.