Altered bladder function in transgenic mice expressing rat elastin.

摘要: The elasticity of tissues subjected to repeated deformation is provided by the presence elastic fibers in extracellular matrix (ECM). most abundant component elastin, whose soluble precursor tropoelastin. To establish role elastin plays bladder, this study describes biosynthetic, histologic, and physiologic consequences expression an isoform rat tropoelastin transgenic mouse bladder. polymerase chain reaction (PCR) was used determine a minigene mice. Histochemical methods were demonstrate changes frozen sections Cystometric analysis carried out non-transgenic mice, prior after 3 weeks partial outlet obstruction. PCR assay demonstrated that bladder tissue mice expressed mRNA, whereas non-transgenes did not. Increased deposition with Verhoeff-van Gieson stain. Bladders animals more compliant than bladders their littermates. Partial obstruction resulted increased volume In contrast, compliance transgenes almost unchanged This demonstrates normal fiber assembly prerequisite for properties wall. Moreover, response critically influenced synthesis. Neurourol. Urodynam. 18:55–68, 1999. © 1999 Wiley-Liss, Inc.