Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
作者： Caitlin Ann Routhier , Mark C. Mochel , Kerry Lynch , Dora Dias-Santagata , David N. Louis
DOI: 10.1016/J.HUMPATH.2013.06.018
关键词: Thyroid carcinoma 、 Melanoma 、 Monoclonal antibody 、 Colorectal cancer 、 Carcinoma 、 Pathology 、 Monoclonal 、 Glioma 、 Immunohistochemistry 、 Cancer research 、 Medicine
摘要: BRAF mutation is seen in a variety of human neoplasms including cutaneous malignant melanoma, papillary thyroid carcinoma, colorectal non-small cell lung pleomorphic xanthoastrocytoma, and others. Currently, there are 2 commercially available monoclonal antibodies for the detection V600E mutation; however, full practical comparison their performance various tumor types on an automated staining platform has not been done. We investigated sensitivity specificity detecting series 152 tumors 31 melanomas, 25 carcinomas, 32 gastrointestinal 23 35 gliomas, 6 other malignancies. In this series, concordance rate between immunohistochemistry (IHC) mutational analyses was 97% (148/152) VE1 88% (131/149) anti-B-Raf. The were 98% (60/61) (88/91) 95% (58/61) 83% (73/88) anti-B-Raf, respectively. There 4 cases with discordant IHC results contrast to 18 Our studies showed that better compared anti-B-Raf confirmed clone provides excellent clinical setting.
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