Sensitive detection of staphylococcal enterotoxin B (SEB) using quantum dots by various methods with special emphasis on an electrochemical immunoassay approach

摘要: Staphylococcal enterotoxin B (SEB) is a potent foodborne pathogen and categorized as class type of biological warfare agent. In this research work, SEB detected by various sensitive analytical methods such enzyme linked immunosorbent assay (ELISA), quantum dots-based fluorescence (QDs-FLISA) square-wave voltammetry (SWV). The obtained results were compared in terms sensitivity, ease experimentation analysis time. For the QD-based detection, fluorescent lead sulfide (PbS) QDs prepared bottom-up approach characterized techniques. Highly specific antibodies against conjugated with PbS used revealing antibodies. electrochemical detection SEB, rabbit anti-SEB polyclonal (primary antibodies) immobilized on screen-printed electrodes (SPEs) followed addition concentrations antigen. These further incubated Finally, 1 M HCl solution was added to SPE dissolve which captured sandwiched immunoassay, resulting Pb2+ ions determined SWV method using glassy-carbon electrode. peak current proportional amount indirectly depends concentration. Linearity observed concentration range ng mL−1 μg limit found be 0.01 for SEB. reveal that sensing much easier, faster provides high sensitivity other methods. It limits achieved sandwich ELISA QDs-FLISA 0.24 0.03 respectively. addition, developed can implemented on-site particularly civil defense applications where security prime importance.