Intratumoral gene therapy of malignant brain tumor in a rat model with angiostatin delivered by adeno-associated viral (AAV) vector.

摘要: We have utilized a recombinant adeno-associated viral (AAV) vector carrying the angiostatin gene as an anti-angiogenesis strategy to treat malignant brain tumor in C6 glioma/Wistar rat model. Angiostatin, potent angiogenesis inhibitor, shows high promises anti-cancer drug through inhibition of neovessel formation. However, sustained vivo protein delivery is required achieve therapeutic effects. The AAV has been proven be able deliver and high-level expression vivo, therefore, well suited such purpose. In this study, we implanted 5 x 10(5) glioma cells into 7 days before therapy. Intratumoral injection high-titer AAV-angiostatin rendered efficacious suppression resulted long-term survival 40% treated rats, whereas control AAV-GFP did not any benefits. addition, investigated combined therapy adenoviral suicidal thymidine kinase along with vector. offered best tumor-suppressive effects increased 55% rats. Our study demonstrated potential using safe effective for anti-angiogenic tumors.