Characterization of morphologic changes and lymphocyte subset distribution in lymph nodes from cats with naturally acquired feline immunodeficiency virus infection.

摘要: Lymph nodes were collected at biopsy or necropsy from 18 cats with naturally acquired symp- tomatic feline immunodeficiency virus (FIV) infection and seronegative cats. Thirty-five of the domestic shorthairs one was a Persian cross. The ranged 7 months to 16 years age mainly obtained California veterinary practitioners, cattery, Veterinary Teaching Hospital. Based on clinical signs present tissue collection, ten FIV-infected fell into im- munodeficiency syndrome (AIDS)-related complex (ARC) stage eight in terminal (AIDS) FIV disease. All FeLV negative by antigen ELISA. Histologic sections lymph each cat examined blindly categorized as hyperplastic, involuting, mixed hyperplastic depleted, normal based upon subjective evaluation follicles paracortex. relative abundance plasma cells evaluated methyl green pyronin (MGP) hematoxylin eosin-stained sections. Similar numbers FIV-seropositive -seronegative node category. only difference evident between control degree plasmacytosis present; moderate marked 13/18 but 3/18 Immunohisto- chemical staining frozen six 12 for CD4, CD8, Factor VIII-related antigen, B cell/follicular dendritic cell-specific (CD2 1 -like) revealed slight increase proportion paracortical CD8+ lymphocytes versus CD4+ These findings suggest that changes previously considered be specific highly suggestive lentivirus-induced disease are actually not occur FIV-seronegative well. Despite this lack specificity, seen may have prognostic significance. ARC had involuting nodes, whereas those AIDS either depleted which suggests correlates category change. Lymphadenopathy occurs early course hu- provide insight mechanisms immunode- man (HIV) is ficiency followed well-Characterized progression (AIDS). architecture lymphocytic subset distribution Feline (FIV), lentivirus n0de~.~,~,'~,~~, ~~,~~,~~~~,~9,~~ include causing an similar exuberant follicular hyperplasia pleo- many respects human AIDS,23,30,35 also induces morphism, progressive involu- alterations. altera- tion depletion. Intrusion CD8 + tions occurring experimentally infected prominent feature during been briefly described,3s recent paper described involution stages.6J1J6 progres- 17 infec- sive alterations parallel tion;'O however, study did tis- HIV diseaseL2 sug- sues comparison. purpose gestive infe~tion.~' Consequently, these characterize morphologic immunohistochem- diagnostic significance ical large