miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas

摘要: In the current setting, we attempted to verify and validate miRNA candidates relevant pediatric primary brain tumor progression outcome, in order provide data regarding identification of novel prognostic biomarkers. Overall, 26 resected tumors were studied from children diagnosed with pilocytic astrocytomas (PAs) (n = 19) ependymomas (EPs) (n = 7). As controls, deceased who underwent autopsy not present any malignancy used. The experimental approach included microarrays covering 1211 miRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed expression profiles miR-15a miR-24-1. multiparameter analyses MATLAB. Matching differentially expressed miRNAs detected both PAs EPs, following distinct comparisons control cohort; however, several cases, they exhibited tissue-specific profiles. On correlations between EP or miR-24-1 found upregulated relapsed cases when compared clinical remission survivors, respectively. Taken together, associations diverse parameters, study repeatedly highlighted as candidate oncogenic molecules associated inferior prognosis ependymoma.