An in vitro model of the inhibition of axon growth in the lesion scar formed after central nervous system injury
作者： Junko Kimura-Kuroda , Xichuan Teng , Yukari Komuta , Nozomu Yoshioka , Kazunori Sango
DOI: 10.1016/J.MCN.2009.10.008
关键词: Neurite 、 Biology 、 Astrocyte 、 Cell biology 、 Extracellular matrix 、 Semaphorin 、 Central nervous system 、 Lesion 、 Glial scar 、 Neuroscience 、 Fibroblast
摘要: After central nervous system (CNS) injury, meningeal fibroblasts migrate in the lesion center to form a fibrotic scar which is surrounded by end feet of reactive astrocytes. The expresses various axonal growth-inhibitory molecules and creates major impediment for regeneration. We developed an vitro model using coculture cerebral astrocytes adding transforming growth factor-beta1 (TGF-beta1), potent fibrogenic factor. Addition TGF-beta1 this resulted enhanced proliferation formation cell clusters consisted inside cluster culture densely accumulated extracellular matrix similar scar, remarkably inhibited neurite outgrowth cerebellar neurons. Therefore, can be available analyze inhibitory property site CNS.
elsevier.com
sci-hub.se 参考文章(63)
V.M. Kähäri, H. Larjava, J. Uitto, Differential regulation of extracellular matrix proteoglycan (PG) gene expression. Transforming growth factor-beta 1 up-regulates biglycan (PGI), and versican (large fibroblast PG) but down-regulates decorin (PGII) mRNA levels in human fibroblasts in culture. Journal of Biological Chemistry. ,vol. 266, pp. 10608- 10615 ,(1991) , 10.1016/S0021-9258(18)99267-7
JS Rudge, J Silver, Inhibition of neurite outgrowth on astroglial scars in vitro The Journal of Neuroscience. ,vol. 10, pp. 3594- 3603 ,(1990) , 10.1523/JNEUROSCI.10-11-03594.1990
Liza Q. Bundesen, Tracy Aber Scheel, Barbara S. Bregman, Lawrence F. Kromer, Ephrin-B2 and EphB2 Regulation of Astrocyte-Meningeal Fibroblast Interactions in Response to Spinal Cord Lesions in Adult Rats The Journal of Neuroscience. ,vol. 23, pp. 7789- 7800 ,(2003) , 10.1523/JNEUROSCI.23-21-07789.2003
Daniel A. Morgenstern, Richard A. Asher, James W. Fawcett, Chapter 22 Chondroitin sulphate proteoglycans in the CNS injury response Progress in Brain Research. ,vol. 137, pp. 313- 332 ,(2002) , 10.1016/S0079-6123(02)37024-9
Richard A. Asher, Daniel A. Morgenstern, Penny S. Fidler, Kathryn H. Adcock, Atsuhiko Oohira, Janet E. Braistead, Joel M. Levine, Richard U. Margolis, John H. Rogers, James W. Fawcett, Neurocan is upregulated in injured brain and in cytokine-treated astrocytes. The Journal of Neuroscience. ,vol. 20, pp. 2427- 2438 ,(2000) , 10.1523/JNEUROSCI.20-07-02427.2000
Carola A. Haas, Uwe Rauch, Niklas Thon, Tobias Merten, Thomas Deller, Entorhinal Cortex Lesion in Adult Rats Induces the Expression of the Neuronal Chondroitin Sulfate Proteoglycan Neurocan in Reactive Astrocytes The Journal of Neuroscience. ,vol. 19, pp. 9953- 9963 ,(1999) , 10.1523/JNEUROSCI.19-22-09953.1999
Nicole Klapka, Hans Werner Müller, Collagen Matrix in Spinal Cord Injury Journal of Neurotrauma. ,vol. 23, pp. 422- 435 ,(2006) , 10.1089/NEU.2006.23.422
Ann Logan, Martin Berry, Ana Maria Gonzalez, Sally A. Frautschy, Michael B. Sporn, Andrew Baird, Effects of transforming growth factor beta 1 on scar production in the injured central nervous system of the rat. European Journal of Neuroscience. ,vol. 6, pp. 355- 363 ,(1994) , 10.1111/J.1460-9568.1994.TB00278.X
Isao Nagata, Katsuhiko Ono, Akio Kawana, Junko Kimura-Kuroda, Aligned neurite bundles of granule cells regulate orientation of Purkinje cell dendrites by perpendicular contact guidance in two-dimensional and three-dimensional mouse cerebellar cultures. The Journal of Comparative Neurology. ,vol. 499, pp. 274- 289 ,(2006) , 10.1002/CNE.21102
Glenn Yiu, Zhigang He, Glial inhibition of CNS axon regeneration Nature Reviews Neuroscience. ,vol. 7, pp. 617- 627 ,(2006) , 10.1038/NRN1956