Absorption, Steady-State Fluorescence, Fluorescence Lifetime, and 2D Self-Assembly Properties of Engineered Fluorescent S-Layer Fusion Proteins of Geobacillus stearothermophilus NRS 2004/3a

摘要: S-layer fusion protein technology was used to design four different fluorescent proteins with three GFP mutants and the red mRFP1. Their absorption spectra, steady-state fluorescence, fluorescence lifetime were investigated as a function of pH. It found that intensities mutant decreased about 50% between pH 6 5. The spectral properties minimally affected by variations. These results compared His-tagged reference proteins, demonstrating did not change general whole protein. In addition, pKa values calculated. Finally, it shown able self-assemble forming 2D nanostructures oblique p2 symmetry lattice parameters = 11 nm, b 14 γ 80°. fluorescenc...