Anisotropic Crystalline Protein Nanolayers as Multi-Functional Biointerface for Patterned Co-Cultures of Adherent and Non-Adherent Cells in Microfluidic Devices

摘要: The spatial arrangement of cells in their microenvironment is known to significantly influence cellular behavior, thus making the control organization an important parameter vitro co-culture models. However, recent advances micropatterning methods within biochips do not address simultaneous cultivation anchorage-dependent and non-adherent cells. To this methodological gap we combine S-layer technology with microfluidics pattern co-cultures study cell-to-cell cell-to-surface interactions under physiologically relevant conditions. We exploit unique self-assembly properties SbpA SbsB S-layers create anisotropic protein nanobiointerface on-chip spatially-defined cytophilic (adhesive) cytophobic (repulsive) properties. While physical parameters such as shear force flow velocities, our regulates biological aspects method including biocompatibility, biostability, affinity reliability reproducibility microfluidic strategy based on laminar patterned nanolayers envisioned advance models for biomedical research.