Gene therapy of rat C6 glioma using adenovirus-mediated transfer of the herpes simplex virus thymidine kinase gene: long-term follow-up by magnetic resonance imaging.

摘要: The herpes simplex virus thymidine kinase gene was transferred into C6 glioma cells by infection with a recombinant adenovirus. In vitro, 10 microM ganciclovir concentration able to kill 100% of the infected cells. For in vivo experiments, brain tumors were established stereotactic injection caudate nucleus rats. Five days later, adenovirus inoculated and animals treated intraperitoneal injections for 14 days. At end therapy, histological examination revealed 28-fold decrease tumor volumes animals, as compared control animals. long-term studies, mean survival time four-fold longer than that ones. Magnetic resonance imaging demonstrated an apparent complete regression 62% However, late recurrence observed Repeated inoculation contralateral hemisphere surviving resulted either rejection or slowly growing tumors. These findings demonstrate potential efficacy adenovirus-mediated transfer administration treatment rat gliomas.