Paenibacillus larvae-Directed Bacteriophage HB10c2 and Its Application in American Foulbrood-Affected Honey Bee Larvae.
作者： Hannes Beims , Johannes Wittmann , Boyke Bunk , Cathrin Spröer , Christine Rohde
DOI: 10.1128/AEM.00804-15
关键词:
摘要: Paenibacillus larvae is the causative agent of American foulbrood (AFB), most serious honey bee brood bacterial disease. We isolated and characterized P. larvae-directed bacteriophages developed criteria for safe phage therapy. Whole-genome analysis a highly lytic virus family Siphoviridae (HB10c2) provided detailed safety profile uncovered its lysogenic nature putative beta-lactamase-like protein. To rate antagonistic activity against pathogens targeted to specify potentially harmful effects on population environment, genotypes ERIC I IV, representatives gut microbiota, broad panel members order Bacillales were analyzed HB10c2-induced lysis. Breeding assays with infected revealed that in vitro observed was not predictive real-life scenario therapeutic efficacy. On basis disclosed larvae-bacteriophage coevolution, we discuss future prospects AFB
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