The isolation, by differential antibody screening, of Echinococcus multilocularis antigen gene clones with potential for immunodiagnosis.

摘要: A λgt11 cDNA expression library consisting of a million recombinant clones has been constructed from Echinococcus multilocularis protoscolex mRNA. Differential screening the with pools E. and granulosus human infection sera revealed 13 potentially immunodiagnostic clones. On basis plaque immunoassays lysogen characteristics, two these clones, designated EM2 EM4, have further characterised. The fusion-peptides purified their potential as reagents assessed by immunoblotting and, in case one fusion-peptide (EM4), enzyme-linked immunosorbent assay (ELISA). Furthermore, native parasite antigens coded for identified. corresponds to 70 kDa protein epitopes EM4 found on three 62, 49 44 kDa. both are present those corresponding appear be excreted/secreted products. They not recognised ELISA variety parasitic other than taken patients infected multilocularis. Nevertheless, protoscoleces Taenia solium cysticerci. These detectable cyst fluid, this may, part, explain lack immune response them T. infections.