GAS5 suppresses malignancy of human glioma stem cells via a miR-196a-5p/FOXO1 feedback loop.

摘要: Glioma stem cells (GSCs) make up highly tumorigenic subpopulations within gliomas, and aberrant expression of GSC genes is a major underlying cause glioma pathogenesis treatment failure. The present study characterized the function long non-coding RNA growth arrest specific 5 (GAS5) in GSCs order to elucidate molecular mechanisms by which GAS5 contributes pathogenesis. We demonstrate that suppresses malignancy binding miR-196a-5p. miR-196a-5p, an onco-miRNA, stimulates proliferation, migration, invasion, addition reducing levels apoptosis. miR-196a-5p specifically downregulates forkhead box protein O1 (FOXO1) targeting its 3' untranslated region (3'-UTR). FOXO1 upregulates phosphotyrosine interaction domain containing 1 (PID1), thereby inhibiting tumorigenicity growth. also migration invasion inhibitory (MIIP), resulting attenuation activities. Interestingly, we show promotes transcription, thus forminga positive feedback loop. These data provide insights into potential new pathways for therapy suggest may be efficacious target treatments.