Researching on new species of "Mate": Ilex brevicuspis: phytochemical and pharmacology study.

摘要: Background: Ilex paraguariensis St. Hilaire (Aquifoliaceae) (“Mate” or “Yerba mate”) is one of the most commercialized plants South America which grows naturally in NE Argentina, Uruguay, SE Brazil and E Paraguay, where it also cultivated. It used to prepare a tea-like beverage (infusions decoctions) appreciated for its peculiar flavor, stimulation nutritional properties. brevicuspis Reisseck same habitat widely as substitute adulterant paraguariensis. In previous work, methylxanthines (caffeine, theobromine theophylline) were not detected by HPLC. Aim study: This study was undertaken order isolate, identify quantify polyphenolic compounds (caffeoyl derivatives flavonoids) investigate some pharmacological activities I. brevicuspis, related with traditional use “Mate” (choleretic, intestinal propulsion antioxidant activities). Acute toxicity investigated. Methods: Decoctions, like extracts, prepared compare results preparations commonly local people. For phytochemical analysis, extracts analyzed HPLC diode array detector. Choleretic assayed rats. Sodium dehydrocholate (DHC) choleretic reference standard. Antioxidant activity tested liposomes that oxidized free radical generator 2,2'-azobis [amidinopropane] chloride (AAPH). Results: first time following isolated quantified: A) caffeoyl derivative (chlorogenic acid; caffeic 3,4-dicaffeoylquinic 3,5-dicaffeoylquinic acid 4,5-dicaffeoylquinic acid. B) flavonoids (rutin, quercetin kaempferol). Biological assays demonstrated produced significant increase bile flow (BF) rats 30 min period percentage BF accumulated at 120 min. an activity. Moreover, this species showed high The acute test did produce any sign doses. Conclusions: An Argentinean specie (I. brevicuspis) has choleretic, propulsion, these may lead potential development new Mate” and/or phytopharmaceutical products, without central nervous system (CNS) stimulant