Identification of Transcriptional Targets of HOXA5

摘要: The homeobox gene HOXA5 encodes a transcription factor that has been shown to play important roles in embryogenesis, hematopoiesis, and tumorigenesis. In order decipher downstream signaling pathways of HOXA5, we utilized oligonucleotide microarray analysis identify genes are differentially expressed HOXA5-induced cells compared with uninduced cells. Comparative expression changes after 9 h induction Hs578T breast cancer identified 306 whose was modulated at least 2-fold. Ten these were also up-regulated by 2-fold 6 post-induction. all 10 confirmed semiquantitative reverse transcription-PCR. Among genes, which most likely be direct targets initiated an investigation into the pleiotrophin first cloning its promoter. Transient transfection assays indicated can specifically activate Promoter deletion, chromatin immunoprecipitation assay, gel-shift performed show directly bind one binding site on These data strongly suggest successfully many potential HOXA5. Further functional will allow us better understand diverse functions embryonic development