Humoral immune responses to phocine herpesvirus-1 in Pacific harbor seals (Phoca vitulina richardsii) during an outbreak of clinical disease.
作者： Donald P. King , Andrew R. Lie , Tracey Goldstein , Brian M. Aldridge , Frances M.D. Gulland
DOI: 10.1016/S0378-1135(00)00369-2
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摘要: Abstract Infection with phocine herpesvirus type-1 (PHV-1) has been associated morbidity and high mortality in neonatal harbor seals ( Phoca vitulina ). A PHV-1 specific indirect enzyme linked immunosorbent assay (ELISA) was developed to sequentially measure the serological status of 106 seal neonates admitted a Pacific coast rehabilitation center (total number sera tested 371). Early season (February–April), majority pups had low serum levels antibody. dramatic increase antibody, involving hospitalized pups, observed during 4-week period May. This coincided incidence adrenal lesions mortality. Although there overall agreement between timing seroconversion histological evidence infection, 82.4% individual adrenalitis no humoral response at time their death. suggests either rapid disease course, or an inability develop some seals.
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