Determination of clenbuterol in bovine liver by enzyme immunoassay
作者： S.D. Bucknall , A.L. MacKenzie , M.J. Sauer , D.J. Everest , R. Newman
DOI: 10.1016/0003-2670(93)80296-W
关键词:
摘要: Abstract Procedures are described for optimising and validating an ELISA method measuring clenbuterol residues in bovine liver without prior sample enrichment. Optimal assay conditions obtained by preincubating supernatant with immobilised antibody raised to clenbuterol-ovalbumen. After further incubation a salbutamol-peroxidase conjugate, colour is developed using tetramethylbenzidene substrate. The will permit measurement of at the maximum residue level 0.5 ng g −1 confidence > 99%.
elsevier.com
sci-hub.se 参考文章(4)
R.J.H. Pickett, M.J. Sauer, Determination of clenbuterol in bovine urine by enzyme immunoassay following concentration and clean-up by immunoaffinity chromatography Analytica Chimica Acta. ,vol. 275, pp. 269- 273 ,(1993) , 10.1016/0003-2670(93)80301-Z
Normand Beaulieu, Claude Charette, Jack C.K. Loo, Nicole Jordan, Iain J. McGilveray, Salbutamol radioimmunoassay: synthesis and properties of the benzylic succinate of salbutamol. Journal of Pharmaceutical and Biomedical Analysis. ,vol. 3, pp. 575- 579 ,(1985) , 10.1016/0731-7085(85)80075-3
Bernard F. Erlanger, [4] The preparation of antigenic Hapten-Carrier conjugates: A survey Methods in Enzymology. ,vol. 70, pp. 85- 104 ,(1980) , 10.1016/S0076-6879(80)70043-5
J.D. Conradie, M. Govender, L. Visser, ELISA solid phase: Partial denaturation of coating antibody yields a more efficient solid phase Journal of Immunological Methods. ,vol. 59, pp. 289- 299 ,(1983) , 10.1016/0022-1759(83)90190-4