Molecular cloning and expression of the hepatitis delta virus genotype IIb genome

摘要: Analysis of hepatitis delta virus (HDV) genome sequences has revealed multiple genotypes with different geographical distributions and associated disease patterns. To date, replication-competent cDNA clones HDV I, II, III have been reported. IIb found in Taiwan. Although full-length genotype published, its replication competence cultured cells yet to be In order examine this, we obtained a clone, Taiwan-IIb-1, from Taiwanese isolate. Comparison the complete nucleic acid sequence Taiwan-IIb-1 previously published isolates indicated that shares 98% identity another isolate 92% Japanese Transfection into COS7 resulted accumulation appearance antigens, showing cloned can replicate cells.