Identification of subpopulations of mononuclear cells in cutaneous infiltrates. I. Differentiation between B cells, T cells, and histiocytes.

摘要: Differentiation between bone marrow-derived lymphocytes (B cells), thymus-derived (T and histiocytes has been achieved in tissue sections through utilization of known differences cell membrane receptors. Sheep erythrocytes (E) were coated with either IgG or IgM antibody (A) to form EA. EA was incubated mouse complement (C) EAC. Cryosections layered 1gM EAC, EA, lgG subpopulations mononuclear cells could be distinguished from one another by the adherence nonadherence these reagents. B bound but not 1gG EA; EAC T failed bind any identified a cutaneous leukemic infiltrate patient leukemia. Histiocytes infiltrating dermis epidermis graft -versus- host reaction. A dermal lymphocytic squamous carcinoma lesion did The remainder fresh this finely minced filtered glass wool column. filtrate positive for human thymic lymphocyte antigen, establishing their identity. It is therefore possible distinguish cells, skin lesions, method should valuable investigation infiltrates identification effector cells.